Find commercial sources for chemical compounds — PubChem/ChEMBL identity resolution then vendor catalog search across ZINC, Enamine, eMolecules, Mcule. Compares pricing, availability, and identifies purchasable analogs when an exact compound is not in stock. Use for chemical procurement, virtual library curation, and 'where can I buy X' questions for synthesis planning.

Connect GWAS variants to biological pathways and druggable targets. Maps GWAS hits to causal genes (via fine-mapping/eQTL), then to pathways (Reactome, KEGG, WikiPathways), then to existing drugs hitting those pathways. Use for pathway-level disease mechanisms, druggable-pathway prioritization from GWAS, SNP-to-pathway-to-target tracing, and tissue-specific eQTL evidence for drug target hypotheses.

Molecular cloning, in both directions. DESIGN — Gibson Assembly (overlap design for seamless multi-fragment joining) and Golden Gate Assembly (Type IIS / BsaI / BbsI / Esp3I / BsmBI / SapI design with unique 4-bp fusion overhangs). ANALYSIS — work out what an existing reaction produces: given input plasmid sequences and an enzyme, digest them, join the fragments by their overhangs, and identify features of the product (expressed ORF, gRNA spacer and its target gene). Use when you need to plan how to join DNA fragments into a construct, design assembly overlaps/overhangs, decide between cloning methods, or determine the product of a stated Gibson/Golden Gate reaction. Covers the domestication (internal-site removal), overhang-uniqueness, and overlap-Tm rules. For PCR primers to generate the fragments, see tooluniverse-primer-design.

Diagnostic test / biomarker accuracy — sensitivity, specificity, PPV, NPV, likelihood ratios, accuracy from a 2x2 table; ROC curve, AUC, and the optimal cutoff (Youden) for a continuous biomarker; and post-test probability via Bayes. Use when you have test results vs a gold standard (binary 2x2, or a continuous score + true labels) and need to judge how good the test is, pick a threshold, or compute the probability of disease given a result. Emphasizes the prevalence-dependence of PPV/NPV.

Review existing work against the user's actual goal and surface evidence-backed strengths, gaps, risks, and next fixes. Use when asked to eval, evaluate, review, assess, or check current/this/my/our work; decide whether a task is complete; build a definition-of-done checklist or rubric; or perform grading, LLM-as-judge, Qworld, or RET evaluation. Treat plain eval/review requests as qualitative: resolve "current work" from the conversation, artifacts, files, or diff, and never assign numeric scores unless the user explicitly requests scores, grades, points, ratings, weighted criteria, Qworld, or RET. Do not use for implementing automated eval suites, tests, graders, or benchmarks.

Genome-ASSEMBLY discovery, QC, and replicon mapping for any organism (bacteria, archaea, fungi, and beyond) using NCBI Datasets. Resolves an organism name or taxid to assemblies, picks the reference/representative or best-quality assembly, pulls assembly QC metrics (total length, contig/scaffold N50, contig count, GC%, assembly level, RefSeq category), enumerates chromosomes and plasmids via per-replicon sequence reports, and compares candidate assemblies on quality. Use for "what genomes are available for [organism]", "assembly stats / N50 / GC content for [GCF_/GCA_ accession]", "how many plasmids does [strain] have", "compare assemblies for [species]", "find the reference genome for [taxon]", "is this assembly Complete Genome or just contigs". NOT for gene-level orthology/synteny (use tooluniverse-comparative-genomics), plant gene structure (use tooluniverse-plant-genomics), de novo assembly from raw reads (no tool exists), or taxonomy-only name/lineage lookups.

Metabolomics pathway analysis — metabolite identification (HMDB, KEGG, ChEBI), pathway mapping (Reactome, KEGG, MetaCyc), disease associations, enzyme/gene linkage. Use for metabolite-to-pathway-to-disease connections, BridgeDb-based ID conversion, and integrating metabolomics with gene-level pathway analyses.

Use when defining or editing MPS language constraints — property validators / setters / getters, referent search scopes (imperative or inherited via `ScopeProvider.getScope`), `referentSetHandler` side effects, default-scope blocks, `canBeChild` / `canBeParent` / `canBeAncestor` / `canBeRoot` placement rules, `defaultConcreteConcept` for abstract concepts, `set <read-only>` and `{name}` aliasing, and scope helpers (`SimpleRoleScope`, `ListScope`, `CompositeScope`, `HidingByNameScope`). Reach for this skill whenever the task involves authoring or modifying `<lang>/languageModels/constraints.mps`.

When the user wants to set up, debug, or interpret app install attribution — including SKAdNetwork (SKAN), Apple's AdAttributionKit, Google Play Install Referrer, MMPs (AppsFlyer, Adjust, Singular, Branch, Kochava), deep links, deferred deep links, conversion values, postback windows, or privacy thresholds. Use when the user mentions "SKAdNetwork", "SKAN", "SKAN 4", "AdAttributionKit", "AAK", "MMP", "AppsFlyer", "Adjust", "Singular", "Branch", "attribution", "conversion value", "postback", "Install Referrer", "deferred deep link", "iOS 14.5", "ATT", "App Tracking Transparency", "IDFA", or "I can't measure my ad campaigns". For paid campaign strategy, see ua-campaign and apple-search-ads. For analytics events, see app-analytics.

When the user wants to optimize their App Store listing for seasonal events, holidays, or trending moments — including keyword opportunities, metadata updates, screenshot theming, and timing strategy. Use when the user mentions "seasonal", "holiday", "Christmas", "New Year", "Valentine's Day", "summer", "back to school", "seasonal keywords", "trending now", "limited time", or wants to capitalize on a calendar event. For general keyword research, see keyword-research. For full metadata rewrites, see metadata-optimization.

When the user wants to design, test, or improve their app icon to increase tap-through rate and conversions in App Store search and browse. Use when the user mentions "app icon", "icon design", "icon A/B test", "icon variants", "tap-through rate", "icon conversion", "icon refresh", or wants to know what makes a good app icon. For screenshot optimization, see screenshot-optimization. For full listing A/B tests, see ab-test-store-listing.

Guided journey from a website with traffic it under-converts to a research-driven growth engine that captures more leads, persuades more buyers, sells a sharper offer, and earns referrals. Orchestrates eight skills phase by phase - cro-methodology, scorecard-marketing, storybrand-messaging, made-to-stick, influence-psychology, hundred-million-offers, contagious, one-page-marketing - asking the user questions at every decision point and recording results in the project docs/ folder (WEBSITE.md, OFFER.md, MARKETING.md, GROW-WEBSITE-PLAN.md) so the journey resumes across sessions. Use when the user wants to raise conversion on a site that already has traffic, capture more leads, sharpen a weak offer, add referral loops, or says 'my site gets visitors but nobody buys'. If a broken funnel or usability friction is the real blocker, run improve-website first; if there is no site yet, use create-website; for one leaking flow, conversion-optimization. For one framework in isolation, invoke that skill directly.

Guided journey from a business with lucky months to a repeatable growth engine that produces a forecast. Orchestrates nine skills phase by phase - one-page-marketing, hundred-million-offers, predictable-revenue, contagious, influence-psychology, crossing-the-chasm, cold-start-problem, lean-analytics, negotiation - asking the user questions at every decision point and recording results in the project docs/ folder (MARKETING.md, OFFER.md, METRICS.md, GROW-BUSINESS-PLAN.md) so the journey resumes across sessions. Use when the user wants to build a marketing and sales system, manufacture predictable pipeline, engineer word-of-mouth and referrals, or says 'revenue is real but lumpy and I need growth that repeats'. If the offer, retention, or operations underneath are broken, run improve-business first; with no paying customers yet, start with create-business; when the product itself must carry the growth loops, use grow-app. For one framework in isolation, invoke that skill directly.

Guided journey from an app people sign up for and then quietly abandon to a sealed retention engine with a habit loop, an activated first run, and one metric the whole team trusts. Orchestrates eight skills phase by phase - hooked-ux, improve-retention, continuous-discovery, lean-ux, inspired-product, lean-analytics, microinteractions, drive-motivation - asking the user questions at every decision point and recording results in the project docs/ folder (PRODUCT.md, METRICS.md, GROW-APP-PLAN.md) so the journey resumes across sessions. Use when the user wants to lift activation and retention, design a habit loop, fix a leaky onboarding funnel, or says 'users sign up then disappear'. Do not use to fix broken UX or performance that no engagement mechanic can paper over - run improve-app first; if there is no app yet, use create-app. For one framework in isolation, invoke that skill directly.

Run the full Stitch SDK generation pipeline. Use when a new tool is added, or the SDK needs to be regenerated end-to-end.

Reviews vMCP code changes for known anti-patterns that make the codebase harder to understand or more brittle. Use when reviewing PRs, planning features, or refactoring vMCP code.

Migrate @data-client/rest path strings from path-to-regexp v6 to v8 syntax. Use when upgrading path-to-regexp, updating RestEndpoint.path or resource path strings, or when seeing errors about unexpected ?, +, (, or ) in paths.

Use when writing, editing, or reviewing C# code that uses System.Reactive (Rx.NET); working with `IObservable`, `IObserver`, `Subject`, `BehaviorSubject`, `ReplaySubject`, or `AsyncSubject`; composing operators like `Select`, `Where`, `Merge`, `Switch`, `Publish`, `RefCount`, `Catch`, `Retry`, `ObserveOn`, or `SubscribeOn`; managing subscription lifecycle with `CompositeDisposable`, `SerialDisposable`, or `DisposeWith`; testing time-dependent reactive code with `TestScheduler` from `Microsoft.Reactive.Testing`; designing hot vs cold observables or enforcing the Rx serial-notification contract. Triggers on phrases like "observable stream", "reactive pipeline", "Rx.NET", "subject leak", "OnNext on wrong thread", "test scheduler", or edits touching files that import `System.Reactive`. Do NOT use for plain `IEnumerable` LINQ, `IAsyncEnumerable`, or TPL `Task`-based async.

Runs SQL queries on CloudWatch Logs data exported as Apache Iceberg tables in S3 Tables. Covers VPC Flow Logs, WAF logs, CloudFront access logs, Route 53 resolver logs, Network Firewall logs, EKS audit logs, Verified Access logs, SES logs, VPC Lattice logs, Step Functions logs, NLB access logs, and 20+ other AWS vended data sources. Applies when analyzing network traffic, investigating security incidents, querying exported logs with SQL, enabling S3 Tables integration, configuring log export, correlating logs with other data, or running Athena queries on the aws-cloudwatch table bucket. Trigger phrases: query logs with SQL, analyze logs in Athena, SQL on VPC flow logs, investigate network traffic, run SQL on exported logs, enable S3 Tables for CloudWatch, correlate logs, historical log analysis, set up log querying.

Runs and interprets AWS Resilience Hub v2 failure mode assessments. Covers starting assessments, understanding findings (severity, categories, recommendations), triaging by achievability, working with AI-generated service functions, and resolving findings. Applies when the user wants to run an assessment, review findings, or understand failure modes, or has a specific finding and asks how to resolve, remediate, or fix it. Does not apply to initial setup (use resilience-hub-getting-started) or FIS experiments.