Conciseness

The body is dense with operational detail (parameters, thresholds, fallbacks), but includes unnecessary duplication: the 7-item "KEY PRINCIPLES" list restates the phase workflow that follows, "Databases Integrated" repeats tool info from each phase, and "Limitations" reiterates inline notes (e.g., the 800-residue limit appears in Phase 0, the fallback table, and Limitations). Not 4: the redundancy spans multiple sections, more than minor trimming; not 2: there is no concept padding or fluff — nearly all prose is operationally load-bearing.

Actionability

Fully concrete, executable guidance for an instruction-only skill: exact tool names with exact parameter names and aliases ("qualifier (or alias uniprot_id / uniprot_accession)"), value formats and examples ("P04637 R175H", "P00520"), calibrated interpretation tables (pLDDT/pTM ranges), and explicit decision logic. The scoring note about instruction-only skills applies — no code absence penalty; the guidance is copy-paste actionable.

Workflow Clarity

Clear Phase 0-6 sequence with per-phase objectives, a decision-logic section, a fallback strategies table (error recovery feedback loops), a "Quality Minimums" checklist for the final report, and an explicit validate-before-act checkpoint for the paid Boltz job ("Always estimate cost before submitting... confirm with the user"). Matches the anchor-5 pattern: explicit validation steps, feedback loops, and checklists for a complex process.

Progressive Disclosure

No bundle files exist and everything lives in one ~370-line SKILL.md. Section structure is clear (headers, tables, phase flow), but optional/advanced material that would naturally sit in separate reference files — the Boltz section, the DTU tools section, the Tool Parameter Reference, and the Databases Integrated table — is fully inlined. Matches anchor 3 ("some structure but... content that should be separate is inline"). Not 2: the structure is strong, not minimal, and nothing is deeply nested; not 4: no references are signaled at all, and the optional sections inflate the core file.

Specificity

Lists multiple specific concrete actions with named tools: "ESMFold de novo prediction, AlphaFold database retrieval, experimental structures from RCSB, ProtVar variant impact assessment, ProtParam sequence properties" — comprehensive coverage of the skill's capabilities. Not 4: no gaps in coverage; every capability in the body is named.

Completeness

Explicitly answers both: what — "Protein 3D structure prediction from sequence — ESMFold... ProtParam sequence properties"; when — "Use for structure prediction when no experimental structure exists, fold-confidence scoring, and structure-guided variant interpretation". Concrete trigger phrases in the 'when' clause match the anchor-5 example structure exactly.

Trigger Term Quality

Good natural keywords: "structure prediction", "no experimental structure exists", "fold-confidence scoring", "variant interpretation", "protein 3D structure". Not 5: a few natural user phrasings are missing ("pLDDT", "model this sequence", "predict the fold", " docking"). Not 3: synonyms like "fold" and "experimental structure" are present, so coverage goes beyond merely relevant.

Distinctiveness Conflict Risk

Clear niche (structure prediction from sequence) with distinct triggers, but "AlphaFold database retrieval, experimental structures from RCSB" overlaps semantically with the sibling retrieval skill (tooluniverse-protein-structure-retrieval, referenced in the body) — a retrieval-only request could route here. Not 5 for that overlap; not 3 since the prediction-centric triggers dominate and routing risk is minor.