Operating rules

• Use scripts/ncbi_blast.py for all concrete BLAST work.
• Honor NCBI limits: >=10s between requests and >=60s between polls for the same RID.
• Always surface the RID in the response so the job can be resumed or refetched later.
• If the conversation is long or multiple tool calls have occurred, refetch from the RID instead of trusting older context.
• If a prior turn saved raw output to disk, do not read it back into context unless the user asks for a specific follow-up.

Execution behavior

• Return compact BLAST summaries first.
• Do not paste full JSON2 or long Text alignments into chat by default.
• Default to max_hits=5 and max_queries=5.
• If the user asks for raw output, write it to a file and report the path.
• Only provide Python code when the user explicitly asks for code or execution is unavailable.
• For normal user-facing answers, summarize the script JSON in markdown; if the user explicitly asks for machine-readable output, return the JSON verbatim.

Input

• The script reads one JSON object from stdin.
• action must be one of submit, status, fetch, or run.
• submit and run require program, database, query_fasta, and email (or NCBI_EMAIL).
• status and fetch require rid.
• program must be one of blastn, blastp, blastx, tblastn, or tblastx.
• result_format defaults to json2 for run and fetch.
• tool defaults to NCBI_TOOL, then ncbi-blast-skill.
• max_hits defaults to 5; max_queries defaults to 5.
• hitlist_size defaults to 50; descriptions and alignments default to 5.
• wait_timeout_sec defaults to 900.
• save_raw defaults to false.
• If save_raw=true and raw_output_path is omitted, the script writes to /tmp/ncbi-blast-<rid>.<json|txt>.
• query_fasta may contain multi-FASTA input; compact summaries still cap per-query output with max_hits and max_queries.

Output

• Common success fields: ok, source, action, warnings.
• submit returns rid, rtoe_seconds, and status="SUBMITTED".
• status returns rid, normalized status, and has_hits.
• run and fetch with result_format=json2 return rid, status, has_hits, result_format, query_count_returned, query_count_available, query_summaries_truncated, query_summaries, and raw_output_path.
• Each query_summary contains query_title, hit_count_returned, hit_count_available, truncated, and top_hits.
• Each top_hit contains rank, accession, title, evalue, and bit_score.
• fetch with result_format=text returns text_head capped at 800 characters unless save_raw=true; when save_raw=true, it returns only the artifact path.
• Failures return ok=false, error.code, error.message, and warnings.

Execution

• Run python scripts/ncbi_blast.py.
• If requests is missing, install it once before first use with python -m pip install requests.

echo '{"action":"run","program":"blastp","database":"swissprot","query_fasta":">q1\nMTEYK...","email":"you@example.com"}' | python scripts/ncbi_blast.py

References

• Load references/blast-common-url-api.txt only for parameter details or uncommon BLAST options.
• Do not load references/intent-notes.txt during normal skill execution; it is not runtime guidance.