Conciseness

The body is mostly lean: terse input checklists, command blocks, and gating sentences with no concept explanations Claude already knows. Not anchor 5 because the local runner invocation appears twice (full 'Plugin-Owned Local Paths' version and a partial repeat under 'Local execution run'), and 'Do not depend on development-only eval harness paths in a shared package' is harness-internal phrasing that earns little.

Actionability

Commands are fully executable and copy-paste ready for the common cases: the two local runner invocations with explicit flags, four preflight commands, a test-profile kickoff, and a real-run skeleton with concrete parameter values. Placeholders (samplesheet.csv, GRCh38) are appropriately generic.

Workflow Clarity

The sequence is clear and gated: confirm inputs, dispatch, preflight, test kickoff, real run, then DE only after 'biological replicates, design formula, and contrasts' are confirmed, plus strandedness inference before final counts. Not anchor 5 because this batch pipeline lacks an explicit output-validation checkpoint (e.g., inspect MultiQC/validation artifacts before proceeding to DE), keeping a minor validation gap.

Progressive Disclosure

Well-organized single-file skill with clean sections, no nested references, and no inlined bulk that belongs in separate files; the skill exceeds 50 lines, so the simple-skill exception to anchor 5 does not apply. The external script paths ('plugins/ngs-analysis/scripts/...') are not bundled in references/ or scripts/, so they cannot be verified from the skill directory.

Specificity

The description names the domain ('bulk RNA-seq') and concrete capabilities ('FASTQ-to-count QC', 'count-matrix differential-expression') with specific tooling ('nf-core/rnaseq, STAR, Salmon, featureCounts, MultiQC, and R/Bioconductor'). It stops short of anchor 5 because the only stated action is dispatching/routing rather than several distinct concrete actions, and it does not specify input formats (e.g., FASTQ/BAM) beyond implied ones.

Completeness

The 'what' is clear (dispatch bulk RNA-seq requests to count-QC or DE skills), but there is no 'Use when...' clause or equivalent explicit trigger guidance, which per the judging guidelines caps completeness at 3. Not anchor 4 because the 'when' is not weakly present — it is entirely absent.

Trigger Term Quality

Strong natural domain keywords: 'bulk RNA-seq', 'FASTQ', 'count matrix', 'differential-expression', plus well-known tool names users say ('STAR', 'Salmon', 'MultiQC', 'R/Bioconductor'). Not anchor 5 because common user variations like 'DESeq2', 'gene expression', or file extensions (.fastq, .csv) are absent.

Distinctiveness Conflict Risk

'bulk RNA-seq' is a clear niche with distinct triggers, so it will not fire on unrelated skills. Not anchor 5 because the description covers the same requests as the two child skills it dispatches to ('counts-qc', 'differential-expression'), creating minor overlap risk with those closely related skills.