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tooluniverse-proteomics-analysis

Analyze mass spectrometry proteomics data including protein quantification, differential expression, post-translational modifications (PTMs), and protein-protein interactions. Processes MaxQuant, Spectronaut, DIA-NN, and other MS platform outputs. Performs normalization, statistical analysis, pathway enrichment, and integration with transcriptomics. Use when analyzing proteomics data, comparing protein abundance between conditions, identifying PTM changes, studying protein complexes, integrating protein and RNA data, discovering protein biomarkers, or conducting quantitative proteomics experiments.

63

Quality

75%

Does it follow best practices?

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Adds up to 20 points to the overall score

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SecuritybySnyk

Low

Low-risk findings worth noting

Fix and improve this skill with Tessl

tessl review fix ./skills/bio/tooluniverse-proteomics-analysis/SKILL.md
SKILL.md
Quality
Evals
Security

Quality

Content

50%

Reviews the quality of instructions and guidance provided to agents. Good implementation is clear, handles edge cases, and produces reliable results.

The body is well-structured and grounded in concrete, executable proteomics code, but it is over-long and monolithic, with several incomplete code stubs and no validation checkpoints in the batch workflow. Splitting detailed material into referenced bundle files and tightening the prose would materially improve it.

Suggestions

Tighten conciseness: strip verbose docstrings and tutorial-style comments, and replace the ~65-line illustrative report template (with fabricated numbers) with a short structural outline or a reference file.

Fix actionability gaps: complete the stub functions (analyze_phosphosites, protein_complex_enrichment, integrate_with_multiomics) and replace the 'hypothetical' ToolUniverse tool calls with real, runnable invocations or clearly-marked placeholders.

Add validation checkpoints to the workflow: insert explicit QC gates (e.g. 'only proceed to DE analysis when missing-value rate and sample correlation pass thresholds') between phases to satisfy the batch-operation feedback-loop expectation.

Apply progressive disclosure: move the per-phase code details and report template into files under references/ or scripts/ and point to them from a concise overview in SKILL.md.

DimensionReasoningScore

Conciseness

The ~840-line body contains executable code but is padded with verbose docstrings ('Load MaxQuant proteinGroups.txt file. Returns: DataFrame...'), explanatory comments ('# Assume missing = low abundance (MNAR assumption)'), and a ~65-line illustrative report template with hardcoded example numbers (e.g. '5,432 proteins', 'MYC (log2FC=3.2)') that do not earn their tokens; it is not score 3 because much could be tightened, and not score 1 because the core code is concrete and actionable rather than abstract filler.

2 / 3

Actionability

Most phases give executable Python using real libraries (pandas, scipy, sklearn, statsmodels, networkx), but several blocks are incomplete stubs: `analyze_phosphosites` returns undefined `phospho_results` after a '# ... perform differential analysis ...' ellipsis, `protein_complex_enrichment` is a `pass` body, `integrate_with_multiomics` returns an undefined `integrated_analysis`, and `predict_kinases` calls a self-described 'hypothetical' tool; this matches score 2 (some concrete guidance but incomplete) and is not score 3 because key pieces are pseudocode/stubs.

2 / 3

Workflow Clarity

The eight-phase pipeline is clearly sequenced with an ASCII workflow diagram, but explicit validation checkpoints are absent for a batch analytical operation — phases proceed with implicit assumptions (e.g. 'Should see similar median' guidance) and no 'validate QC before proceeding' gate; per the rubric's batch-operation guidance this caps the score at 2 rather than 3, and it is not 1 because the sequence itself is well laid out.

2 / 3

Progressive Disclosure

No bundle files exist (no references/, scripts/, or assets/) and all content lives inline in one monolithic 840-line SKILL.md, including the full report template and per-phase code that should be split into separate referenced files; it is not score 3 because there are no one-level-deep references to separate materials, and not score 1 because the body is well-organized into labeled sections rather than a poorly organized wall of text.

2 / 3

Total

8

/

12

Passed

Description

100%

Based on the skill's description, can an agent find and select it at the right time? Clear, specific descriptions lead to better discovery.

A strong, third-person description that pairs a comprehensive list of concrete capabilities with an explicit, trigger-rich 'Use when' clause. It clearly communicates both what the skill does and when to invoke it, with minimal overlap risk.

DimensionReasoningScore

Specificity

Lists multiple concrete actions across two sentences — 'protein quantification, differential expression, post-translational modifications (PTMs), and protein-protein interactions' plus 'normalization, statistical analysis, pathway enrichment, and integration with transcriptomics' and named platforms (MaxQuant, Spectronaut, DIA-NN), matching the score-3 anchor of several specific concrete actions; it is not score 2 because the action list is comprehensive rather than partial.

3 / 3

Completeness

It explicitly answers both 'what' (analyze... processes... performs...) and 'when' via an explicit 'Use when' clause, matching the score-3 anchor for clear what-and-when with explicit triggers; it is not score 2 because the when-guidance is explicit rather than implied.

3 / 3

Trigger Term Quality

The 'Use when' clause enumerates natural user phrasings — 'analyzing proteomics data', 'comparing protein abundance', 'identifying PTM changes', 'studying protein complexes', 'integrating protein and RNA data', 'protein biomarkers', 'quantitative proteomics experiments' — giving good coverage of terms a user would actually say; it is not score 2 because it covers common variations rather than just a few relevant keywords.

3 / 3

Distinctiveness Conflict Risk

The proteomics/MS niche with distinctive triggers ('MaxQuant', 'Spectronaut', 'DIA-NN', 'proteomics data', 'protein abundance') is clearly distinguishable from generic genomics or multi-omics skills and unlikely to fire for the wrong skill; it is not score 2 because the triggers are domain-specific rather than broadly overlapping.

3 / 3

Total

12

/

12

Passed

Validation

93%

Checks the skill against the spec for correct structure and formatting. All validation checks must pass before discovery and implementation can be scored.

Validation15 / 16 Passed

Validation for skill structure

CriteriaDescriptionResult

skill_md_line_count

SKILL.md is long (844 lines); consider splitting into references/ and linking

Warning

Total

15

/

16

Passed

Repository
wu-yc/LabClaw
Reviewed

Table of Contents

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